Mechanisms of DNA opening revealed in AAA+ transcription complex structures Fuzhou Ye, Forson Gao, Xiaojiao Liu, Martin Burt, Xiaodong Zhang
Science Advances, 8 Dec 2022 DOI: 10.1126/sciadv.add3479 Electron cryo-microscopy reveals the structure of the archaeal thread filament Matthew C. Gaines, Michail N. Isupov, Shamphavi Sivabalasarma, Risat U. I. Haque, Mathew Mclaren, Clara L. Mollat, Patrick Tripp, Alexander Neuhaus, Vicki A. M. Gold, Sonja-Verena Albers, Bertram Daum
Nature Communications, 13 Dec 2022 DOI: 10.1038/s41467-022-34652-4 A conserved glutathione binding site in poliovirus is a target for antivirals and vaccine stabilisation Mohammad W. Bahar, Veronica Nasta, Helen Fox, Lee Sherry, Keith Grehan, Claudine Porta, Andrew J. Macadam, Nicola J. Stonehouse, David J. Rowlands, Elizabeth E. Fry, David I. Stuart
Communications Biology, 5 Nov 2022 DOI: 10.1038/s42003-022-04252-5 Structural basis of tankyrase activation by polymerization Nisha Pillay, Laura Mariotti, Mariola Zaleska, Oviya Inian, Matthew Jessop, Sam Hibbs, Ambroise Desfosses, Paul C. R. Hopkins, Catherine M. Templeton, Fabienne Beuron, Edward P. Morris, Sebastian Guettler
Nature, 20 Nov 2022 DOI: 10.1038/s41586-022-05449-8 Journal Paper Krios I-Titan Krios I at Diamond Krios II-Titan Krios II at Diamond Krios III-Titan Krios III at Diamond open access CryoEM structure and assembly mechanism of a bacterial virus genome gatekeeper Igor Orlov, Stéphane Roche, Sandrine Brasilès, Natalya Lukoyanova, Marie-Christine Vaney, Paulo Tavares, Elena V. Orlova
Nature Communications, 13 Nov 2022 DOI: 10.1038/s41467-022-34999-8 Cryo‐EM structure of the human NKCC1 transporter reveals mechanisms of ion coupling and specificity Caroline Neumann, Lena Lindtoft Rosenbæk, Rasmus Kock Flygaard, Michael Habeck, Jesper Lykkegaard Karlsen, Yong Wang, Kresten Lindorff-Larsen, Hans Henrik Gad, Rune Hartmann, Joseph A. Lyons, Robert A. Fenton, Poul Nissen
The Embo Journal, 8 Oct 2022 DOI: 10.15252/embj.2021110169 Structural basis for host recognition and superinfection exclusion by bacteriophage T5 Bert Van Den Berg, Augustinas Silale, Arnaud Basle, Astrid F. Brandner, Sophie L. Mader, Syma Khalid
Proceedings Of The National Academy Of Sciences, 119 Oct 2022 DOI: 10.1073/pnas.2211672119 Journal Paper I04-Macromolecular Crystallography Krios II-Titan Krios II at Diamond open access Cryo‐EM structures of perforin‐2 in isolation and assembled on a membrane suggest a mechanism for pore formation Xiulian Yu, Tao Ni, George Munson, Peijun Zhang, Robert J. C. Gilbert
The Embo Journal, 11 Oct 2022 DOI: 10.15252/embj.2022111857 CryoEM structural exploration of catalytically active enzyme pyruvate carboxylase Jorge Pedro Lopez-Alonso, Melisa Lazaro, David Gil-Carton, Philip H. Choi, Liang Tong, Mikel Valle
Nature Communications, 13 Oct 2022 DOI: 10.1038/s41467-022-33987-2 Journal Paper Krios II-Titan Krios II at Diamond Krios III-Titan Krios III at Diamond open access Structure and mechanism of the type I-G CRISPR effector Qilin Shangguan, Shirley Graham, Ramasubramanian Sundaramoorthy, Malcolm F. White
Nucleic Acids Research, 50 Oct 2022 DOI: 10.1093/nar/gkac925 Structure and activity of particulate methane monooxygenase arrays in methanotrophs Yanan Zhu, Christopher W. Koo, C. Keith Cassidy, Matthew C. Spink, Tao Ni, Laura C. Zanetti-Domingues, Benji Bateman, Marisa Martin-Fernandez, Juan Shen, Yuewen Sheng, Yun Song, Zhengyi Yang, Amy C. Rosenzweig, Peijun Zhang
Nature Communications, 13 Sept 2022 DOI: 10.1038/s41467-022-32752-9 Journal Paper Krios I-Titan Krios I at Diamond Krios II-Titan Krios II at Diamond open access The DNA-damage kinase ATR activates the FANCD2-FANCI clamp by priming it for ubiquitination Tamara Sijacki, Pablo Alcon, Zhuo A. Chen, Stephen H. Mclaughlin, Shabih Shakeel, Juri Rappsilber, Lori Passmore
Nature Structural & Molecular Biology, 326 Sept 2022 DOI: 10.1038/s41594-022-00820-9 Structures and mechanism of the plant PIN-FORMED auxin transporter Kien Lam Ung, Mikael Winkler, Lukas Schulz, Martina Kolb, Dorina P. Janacek, Emil Dedic, David L. Stokes, Ulrich Z. Hammes, Bjorn Panyella Pedersen
Nature, 609 Sept 2022 DOI: 10.1038/s41586-022-04883-y Structures of α-synuclein filaments from human brains with Lewy pathology Yang Yang, Yang Shi, Manuel Schweighauser, Xianjun Zhang, Abhay Kotecha, Alexey G. Murzin, Holly J. Garringer, Patrick W. Cullinane, Yuko Saito, Tatiana Foroud, Thomas T. Warner, Kazuko Hasegawa, Ruben Vidal, Shigeo Murayama, Tamas Revesz, Bernardino Ghetti, Masato Hasegawa, Tammaryn Lashley, Sjors H. W. Scheres, Michel Goedert
Nature, 29 Sept 2022 DOI: 10.1038/s41586-022-05319-3 Access denied: Stopping rabies virus from entering cells Diamond Light Source
Sept 2022 Science Highlight Krios I-Titan Krios I at Diamond
Cryo-EM reveals a mechanism of USP1 inhibition through a cryptic binding site Martin L. Rennie, Connor Arkinson, Viduth K. Chaugule, Helen Walden
Science Advances, 8 Sept 2022 DOI: 10.1126/sciadv.abq6353 Structural insights into the assembly and activation of the IL ‐27 signaling complex Yibo Jin, Paul K. Fyfe, Scott Gardner, Stephan Wilmes, Doryen Bubeck, Ignacio Moraga
Embo Reports, Aug 2022 DOI: 10.15252/embr.202255450 Structure and dynamics of Toll immunoreceptor activation in the mosquito Aedes aegypti Yoann Saucereau, Thomas H. Wilson, Matthew C. K. Tang, Martin C. Moncrieffe, Steven W. Hardwick, Dimitri Y. Chirgadze, Sandro G. Soares, Maria Jose Marcaida, Nicholas J. Gay, Monique Gangloff
Nature Communications, 13 Aug 2022 DOI: 10.1038/s41467-022-32690-6 Journal Paper B21-High Throughput SAXS Krios I-Titan Krios I at Diamond open access Structure of trimeric pre-fusion rabies virus glycoprotein in complex with two protective antibodies Weng M. Ng, Sofiya Fedosyuk, Solomon English, Gilles Augusto, Adam Berg, Luke Thorley, Anna-Sophie Haselon, Rameswara R. Segireddy, Thomas A. Bowden, Alexander D. Douglas
Cell Host & Microbe, 58 Aug 2022 DOI: 10.1016/j.chom.2022.07.014 Structure of a nucleosome-bound MuvB transcription factor complex reveals DNA remodelling Marios G. Koliopoulos, Reyhan Muhammad, Theodoros I. Roumeliotis, Fabienne Beuron, Jyoti S. Choudhary, Claudio Alfieri
Nature Communications, 13 Aug 2022 DOI: 10.1038/s41467-022-32798-9 Exploring the molecular mechanisms of Ag(I) transporters involved in bacterial Ag(I) resistance Ryan Lithgo
Aug 2022 Thesis B23-Circular Dichroism I24-Microfocus Macromolecular Crystallography Krios I-Titan Krios I at Diamond
Correlation between the binding affinity and the conformational entropy of nanobody SARS-CoV-2 spike protein complexes Halina Mikolajek, Miriam Weckener, Z. Faidon Brotzakis, Jiandong Huo, Evmorfia V. Dalietou, Audrey Le Bas, Pietro Sormanni, Peter J. Harrison, Philip N. Ward, Steven Truong, Lucile Moynie, Daniel K. Clare, Maud Dumoux, Joshua Dormon, Chelsea Norman, Naveed Hussain, Vinod Vogirala, Raymond J. Owens, Michele Vendruscolo, James Naismith
Proceedings Of The National Academy Of Sciences, 119 Jul 2022 DOI: 10.1073/pnas.2205412119 Journal Paper I03-Macromolecular Crystallography Krios II-Titan Krios II at Diamond Krios IV-Titan Krios IV at Diamond open access Structure of PLA2R reveals presentation of the dominant membranous nephropathy epitope and an immunogenic patch Maryline Fresquet, Michael P. Lockhart-Cairns, Samuel J. Rhoden, Thomas A. Jowitt, David C. Briggs, Clair Baldock, Paul E. Brenchley, Rachel Lennon
Proceedings Of The National Academy Of Sciences, 119 Jul 2022 DOI: 10.1073/pnas.2202209119 Journal Paper B21-High Throughput SAXS Krios IV-Titan Krios IV at Diamond open access Molecular basis for the regulation of human glycogen synthase by phosphorylation and glucose-6-phosphate Thomas J. Mccorvie, Paula M. Loria, Meihua Tu, Seungil Han, Leela Shrestha, D. Sean Froese, Igor M. Ferreira, Allison P. Berg, Wyatt W. Yue
Nature Structural & Molecular Biology, 29 Jul 2022 DOI: 10.1038/s41594-022-00799-3 Cryo‐EM reveals mechanisms of angiotensin I‐converting enzyme allostery and dimerization Lizelle Lubbe, Bryan Trevor Sewell, Jeremy D. Woodward, Edward D. Sturrock
The Embo Journal, 66 Jul 2022 DOI: 10.15252/embj.2021110550 Structure and assembly of cargo Rubisco in two native α-carboxysomes Tao Ni, Yaqi Sun, Will Burn, Monsour M. J. Al-Hazeem, Yanan Zhu, Xiulian Yu, Lu-Ning Liu, Peijun Zhang
Nature Communications, 13 Jul 2022 DOI: 10.1038/s41467-022-32004-w The aggregation of alpha-synuclein and its disaggregation by the Hsp70 disaggregation machinery Jim Emilien Jacques Monistrol
Jul 2022 Thesis Krios I-Titan Krios I at Diamond M01-Polara at OPIC (Oxford)
Cryo-electron tomography remote data collection and subtomogram averaging Yuewen Sheng, Kyle Morris, Julika Radecke, Peijun Zhang
Journal Of Visualized Experiments, Jul 2022 DOI: 10.3791/63923 Cooperative amyloid fibre binding and disassembly by the Hsp70 disaggregase Joseph G. Beton, Jim Monistrol, Anne Wentink, Erin C. Johnston, Anthony J. Roberts, Bernd G. Bukau, Bart W. Hoogenboom, Helen R. Saibil
The Embo Journal, 9 June 2022 DOI: 10.15252/embj.2021110410 Mechanism of mitoribosomal small subunit biogenesis and preinitiation Yuzuru Itoh, Anas Khawaja, Ivan Laptev, Miriam Cipullo, Ilian Atanassov, Petr Sergiev, Joanna Rorbach, Alexey Amunts
Nature, 606 June 2022 DOI: 10.1038/s41586-022-04795-x Cryo-EM structures of the Synechocystis sp. PCC 6803 cytochrome b 6 f complex with and without the regulatory PetP subunit Matthew S Proctor, Lorna A. Malone, David A. Farmer, David J. K. Swainsbury, Frederick R. Hawkings, Federica Pastorelli, Thomas Z. Emrich-Mills, C. Alistair Siebert, C. Neil Hunter, Matthew P. Johnson, Andrew Hitchcock
Biochemical Journal, June 2022 DOI: 10.1042/BCJ20220124 Thicker ice improves the integrity and angular distribution of CDC48A hexamers on Cryo-EM grids Brandon Huntington, Lingyun Zhao, Patrick Bron, Umar F. Shahul Hameed, Stefan T. Arold, Bilal M. Qureshi
Frontiers In Molecular Biosciences, 9 June 2022 DOI: 10.3389/fmolb.2022.890390 Potent cross-reactive antibodies following Omicron breakthrough in vaccinees Rungtiwa Nutalai, Daming Zhou, Aekkachai Tuekprakhon, Helen M. Ginn, Piyada Supasa, Chang Liu, Jiandong Huo, Alexander J. Mentzer, Helen M. E. Duyvesteyn, Aiste Dijokaite-Guraliuc, Donal Skelly, Thomas G. Ritter, Ali Amini, Sagida Bibi, Sandra Adele, Sile Ann Johnson, Bede Constantinides, Hermione Webster, Nigel Temperton, Paul Klenerman, Eleanor Barnes, Susanna J. Dunachie, Derrick Crook, Andrew J. Pollard, Teresa Lambe, Philip Goulder, Neil G. Paterson, Mark A. Williams, David R. Hall, Juthathip Mongkolsapaya, Elizabeth E. Fry, Wanwisa Dejnirattisai, Jingshan Ren, David I. Stuart, Gavin R. Screaton, Christopher Conlon, Alexandra Deeks, John Frater, Lisa Frending, Siobhan Gardiner, Anni Jämsén, Katie Jeffery, Tom Malone, Eloise Phillips, Lucy Rothwell, Lizzie Stafford
Cell, May 2022 DOI: 10.1016/j.cell.2022.05.014 Journal Paper I03-Macromolecular Crystallography I04-Macromolecular Crystallography Krios I-Titan Krios I at Diamond open access Cryo-EM structures show the mechanistic basis of pan-peptidase inhibition by human α2-macroglobulin Daniel Luque, Theodoros Goulas, Carlos P. Mata, Soraia Mendes, F. Xavier Gomis-Rüth, Jose R. Caston
Proceedings Of The National Academy Of Sciences, 119 May 2022 DOI: 10.1073/pnas.2200102119 Cryo-EM structure and rRNA modification sites of a plant ribosome Patrick Cottilli, Yuzuru Itoh, Yuko Nobe, Anton S. Petrov, Purificación Lisón, Masato Taoka, Alexey Amunts
Plant Communications, May 2022 DOI: 10.1016/j.xplc.2022.100342 Mpe1 senses the binding of pre-mRNA and controls 3′ end processing by CPF Juan B. Rodriguez-Molina, Francis J. O'Reilly, Holly Fagarasan, Eleanor Sheekey, Sarah Maslen, J. Mark Skehel, Juri Rappsilber, Lori A. Passmore
Molecular Cell, 577 May 2022 DOI: 10.1016/j.molcel.2022.04.021 The structural basis of Cdc7-Dbf4 kinase dependent targeting and phosphorylation of the MCM2-7 double hexamer Almutasem Saleh, Yasunori Noguchi, Ricardo Aramayo, Marina E. Ivanova, Kathryn M. Stevens, Alex Montoya, Sunidhi Sunidhi, Nicolas Lopez Carranza, Marcin J. Skwark, Christian Speck
Nature Communications, 13 May 2022 DOI: 10.1038/s41467-022-30576-1 Journal Paper Krios I-Titan Krios I at Diamond Krios II-Titan Krios II at Diamond open access A bioactive phlebovirus-like envelope protein in a hookworm endogenous virus Monique Merchant, Carlos P. Mata, Yangci Liu, Haoming Zhai, Anna V. Protasio, Yorgo Modis
Science Advances, 8 May 2022 DOI: 10.1126/sciadv.abj6894 SuRVoS 2: accelerating annotation and segmentation for large volumetric bioimage workflows across modalities and scales Avery Pennington, Oliver N. F. King, Win Min Tun, Elaine M. L. Ho, Imanol Luengo, Michele C. Darrow, Mark Basham
Frontiers In Cell And Developmental Biology, 10 Apr 2022 DOI: 10.3389/fcell.2022.842342 Journal Paper B24-Cryo Soft X-ray Tomography I13-2-Diamond Manchester Imaging Krios I-Titan Krios I at Diamond open access Assessing the mobility of severe acute respiratory syndrome coronavirus-2 spike protein glycans by structural and computational methods Soledad Stagnoli, Francesca Peccati, Sean R. Connell, Ane Martinez-Castillo, Diego Charro, Oscar Millet, Chiara Bruzzone, Asis Palazon, Ana Ardá, Jesús Jiménez-Barbero, June Ereño-Orbea, Nicola G. A. Abrescia, Gonzalo Jiménez-Osés
Frontiers In Microbiology, 13 Apr 2022 DOI: 10.3389/fmicb.2022.870938 Journal Paper Krios II-Titan Krios II at Diamond Krios IV-Titan Krios IV at Diamond open access Structure of the human inner kinetochore bound to a centromeric CENP-A nucleosome Stanislau Yatskevich, Kyle W. Muir, Dom Bellini, Ziguo Zhang, Jing Yang, Thomas Tischer, Masa Predin, Tom Dendooven, Stephen H. Mclaughlin, David Barford
Science, Apr 2022 DOI: 10.1126/science.abn3810 Snapshots of actin and tubulin folding inside the TRiC chaperonin John J. Kelly, Dale Tranter, Els Pardon, Gamma Chi, Holger Kramer, Lotta Happonen, Kelly M. Knee, Jay M. Janz, Jan Steyaert, Christine Bulawa, Ville O. Paavilainen, Juha T. Huiskonen, Wyatt W. Yue
Nature Structural & Molecular Biology, 15 Apr 2022 DOI: 10.1038/s41594-022-00755-1 Cryo-ET detects bundled triple helices but not ladders in meiotic budding yeast Olivia X. Ma, Wen Guan Chong, Joy K. E. Lee, Shujun Cai, C. Alistair Siebert, Andrew Howe, Peijun Zhang, Jian Shi, Uttam Surana, Lu Gan
Plos One, 17 Apr 2022 DOI: 10.1371/journal.pone.0266035 Application of super-resolution and correlative double sampling in cryo-electron microscopy Yuewen Sheng, Peter J. Harrison, Vinod Vogirala, Zhengyi Yang, Claire Strain-Damerell, Thomas Frosio, Benjamin A. Himes, C. Alistair Siebert, Peijun Zhang, Daniel Clare
Faraday Discussions, Apr 2022 DOI: 10.1039/D2FD00049K Journal Paper Krios II-Titan Krios II at Diamond Krios IV-Titan Krios IV at Diamond open access eIF6 rebinding dynamically couples ribosome maturation and translation Pekka Jaako, Alexandre Faille, Shengjiang Tan, Chi C. Wong, Norberto Escudero-Urquijo, Pablo Castro-Hartmann, Penny Wright, Christine Hilcenko, David J. Adams, Alan J. Warren
Nature Communications, 13 Mar 2022 DOI: 10.1038/s41467-022-29214-7 Age-dependent formation of TMEM106B amyloid filaments in human brains Manuel Schweighauser, Diana Arseni, Mehtap Bacioglu, Melissa Huang, Sofia Lovestam, Yang Shi, Yang Yang, Wenjuan Zhang, Abhay Kotecha, Holly J. Garringer, Ruben Vidal, Grace I. Hallin, Kathy L. Newell, Airi Tarutani, Shigeo Murayama, Masayuki Miyazaki, Yuko Saito, Mari Yoshida, Kazuko Hasegawa, Tammaryn Lashley, Tamas Revesz, Gabor G. Kovacs, John Van Swieten, Masaki Takao, Masato Hasegawa, Bernardino Ghetti, Maria Grazia Spillantini, Benjamin Ryskeldi-Falcon, Alexey G. Murzin, Michel Goedert, Sjors H. W. Scheres
Nature, Mar 2022 DOI: 10.1038/s41586-022-04650-z 2.4-Å structure of the double-ring Gemmatimonas phototrophica photosystem Pu Qian, Alastair T. Gardiner, Ivana Šímová, Katerina Naydenova, Tristan I. Croll, Philip J. Jackson, Nupur Nupur, Miroslav Kloz, Petra Čubáková, Marek Kuzma, Yonghui Zeng, Pablo Castro-Hartmann, Bart Van Knippenberg, Kenneth N. Goldie, David Kaftan, Pavel Hrouzek, Jan Hájek, Jon Agirre, C. Alistair Siebert, David Bína, Kasim Sader, Henning Stahlberg, Roman Sobotka, Christopher J. Russo, Tomáš Polívka, C. Neil Hunter, Michal Koblížek
Science Advances, 8 Feb 2022 DOI: 10.1126/sciadv.abk3139 Mechanisms underlying TARP modulation of the GluA1/2-γ8 AMPA receptor Beatriz Herguedas, Bianka K. Kohegyi, Jan-Niklas Dohrke, Jake F. Watson, Danyang Zhang, Hinze Ho, Saher A. Shaikh, Remigijus Lape, James M. Krieger, Ingo H. Greger
Nature Communications, 13 Feb 2022 DOI: 10.1038/s41467-022-28404-7 Structure and assembly of the S-layer in C. difficile Paola Lanzoni-Mangutchi, Oishik Banerji, Jason Wilson, Anna Barwinska-Sendra, Joseph A. Kirk, Filipa Vaz, Shauna O’beirne, Arnaud Basle, Kamel El Omari, Armin Wagner, Neil F. Fairweather, Gillian R. Douce, Per A. Bullough, Robert P. Fagan, Paula Salgado
Nature Communications, 13 Feb 2022 DOI: 10.1038/s41467-022-28196-w Journal Paper I04-Macromolecular Crystallography I23-Long wavelength MX I24-Microfocus Macromolecular Crystallography Krios III-Titan Krios III at Diamond open access A cryo-ET survey of microtubules and intracellular compartments in mammalian axons Helen E. Foster, Camilla Ventura Santos, Andrew P. Carter
Journal Of Cell Biology, 221 Feb 2022 DOI: 10.1083/jcb.202103154 Structure of ATP synthase from ESKAPE pathogen Acinetobacter baumannii Julius K. Demmer, Ben P. Phillips, O. Lisa Uhrig, Alain Filloux, Luke P. Allsopp, Maike Bublitz, Thomas Meier
Science Advances, 8 Feb 2022 DOI: 10.1126/sciadv.abl5966 Journal Paper Krios III-Titan Krios III at Diamond Krios IV-Titan Krios IV at Diamond open access The pore conformation of lymphocyte perforin Marina E. Ivanova, Natalya Lukoyanova, Sony Malhotra, Maya Topf, Joseph A. Trapani, Ilia Voskoboinik, Helen R. Saibil
Science Advances, 8 Feb 2022 DOI: 10.1126/sciadv.abk3147 High-resolution in situ structure determination by cryo-electron tomography and subtomogram averaging using emClarity Tao Ni, Thomas Frosio, Luiza Mendonca, Yuewen Sheng, Daniel Clare, Benjamin A. Himes, Peijun Zhang
Nature Protocols, 58 Jan 2022 DOI: 10.1038/s41596-021-00648-5 Structural mechanism for tyrosine hydroxylase inhibition by dopamine and reactivation by Ser40 phosphorylation Maria Teresa Bueno-Carrasco, Jorge Cuellar, Marte I. Flydal, César Santiago, Trond-André Kråkenes, Rune Kleppe, José R. López-Blanco, Miguel Marcilla, Knut Teigen, Sara Alvira, Pablo Chacón, Aurora Martinez, José Maria Valpuesta
Nature Communications, 13 Jan 2022 DOI: 10.1038/s41467-021-27657-y Cryo-electron microscopy reveals how acetogenins inhibit mitochondrial respiratory complex I Daniel Grba, James N. Blaza, Hannah R. Bridges, Ahmed-Nnoor A. Agip, Zhan Yin, Masatoshi Murai, Hideto Miyoshi, Judy Hirst
Journal Of Biological Chemistry, 82 Jan 2022 DOI: 10.1016/j.jbc.2022.101602 Pore dynamics and asymmetric cargo loading in an encapsulin nanocompartment Jennifer Ross, Zak Mciver, Thomas Lambert, Cecilia Piergentili, Jasmine Emma Bird, Kelly J. Gallagher, Faye L. Cruickshank, Patrick James, Efrain Zarazúa-Arvizu, Louise E. Horsfall, Kevin J. Waldron, Marcus D. Wilson, C. Logan Mackay, Arnaud Basle, David J. Clarke, Jon Marles-Wright
Science Advances, 8 Jan 2022 DOI: 10.1126/sciadv.abj4461