Lipid interactions of a ciliary membrane TRP channel: Simulation and structural studies of polycystin-2 Qinrui Wang, Robin A. Corey, George Hedger, Prafulla Aryal, Mariana Grieben, Chady Nasrallah, Agnese Baronina, Ashley C. W. Pike, Jiye Shi, Elisabeth P. Carpenter, Mark S. P. Sansom
Structure, Dec 2019 DOI: 10.1016/j.str.2019.11.005 Structural basis of nucleosome assembly by the Abo1 AAA+ ATPase histone chaperone Carol Cho, Juwon Jang, Yujin Kang, Hiroki Watanabe, Takayuki Uchihashi, Seung Joong Kim, Koichi Kato, Ja Yil Lee, Ji-Joon Song
Nature Communications, 10 Dec 2019 DOI: 10.1038/s41467-019-13743-9 The cochaperone CHIP marks Hsp70- and Hsp90-bound substrates for degradation through a very flexible mechanism Lucía Quintana-Gallardo, Jaime Martin-Benito, Miguel Marcilla, Guadalupe Espadas, Eduard Sabidó, José María Valpuesta
Scientific Reports, 9 Dec 2019 DOI: 10.1038/s41598-019-41060-0 The cryo-electron microscopy supramolecular structure of the bacterial stressosome unveils its mechanism of activation Allison H. Williams, Adam Redzej, Nathalie Rolhion, Tiago R. D. Costa, Aline Rifflet, Gabriel Waksman, Pascale Cossart
Nature Communications, 10 Dec 2019 DOI: 10.1038/s41467-019-10782-0 Cryo-EM structure of nucleotide-bound Tel1ATM unravels the molecular basis of inhibition and structural rationale for disease-associated mutations Luke A. Yates, Rhys M. Williams, Sarem Hailemariam, Rafael Ayala, Peter Burgers, Xiaodong Zhang
Structure, Nov 2019 DOI: 10.1016/j.str.2019.10.012 The C-terminal dimerization domain of the respiratory mucin MUC5B functions in mucin stability and intracellular packaging before secretion Caroline Ridley, Michael P. Lockhart-Cairns, Richard F. Collins, Thomas A. Jowitt, Durai B. Subramani, Mehmet Kesimer, Clair Baldock, David J. Thornton
Journal Of Biological Chemistry, 294 Nov 2019 DOI: 10.1074/jbc.RA119.010771 Journal Paper B21-High Throughput SAXS Krios I-Titan Krios I at Diamond open access Structure of the Fanconi anaemia monoubiquitin ligase complex Shabih Shakeel, Eeson Rajendra, Pablo Alcon, Francis O'Reilly, Dror S. Chorev, Sarah Maslen, Gianluca Degliesposti, Christopher J. Russo, Shaoda He, Chris H. Hill, J. Mark Skehel, Sjors H. W. Scheres, Ketan J. Patel, Juri Rappsilber, Carol V. Robinson, Lori A. Passmore
Nature, 575 Nov 2019 DOI: 10.1038/s41586-019-1703-4 Mechanism of ribosome stalling during translation of a poly(A) tail Viswanathan Chandrasekaran, Szymon Juszkiewicz, Junhong Choi, Joseph D. Puglisi, Alan Brown, Sichen Shao, V. Ramakrishnan, Ramanujan S. Hegde
Nature Structural & Molecular Biology, 26 Nov 2019 DOI: 10.1038/s41594-019-0331-x Designer enzymes on the way Diamond Light Source
Nov 2019 Science Highlight Krios II-Titan Krios II at Diamond
Structural insight into eukaryotic sterol transport through Niemann-Pick Type C proteins Mikael B. L. Winkler, Rune T. Kidmose, Maria Szomek, Katja Thaysen, Shaun Rawson, Stephen P. Muench, Daniel Wüstner, Bjorn Panyella Pedersen
Cell, Oct 2019 DOI: 10.1016/j.cell.2019.08.038 Journal Paper I04-1-Macromolecular Crystallography (fixed wavelength) I04-Macromolecular Crystallography I24-Microfocus Macromolecular Crystallography Krios I-Titan Krios I at Diamond Krios II-Titan Krios II at Diamond The cryo-EM structure of African swine fever virus unravels a unique architecture comprising two icosahedral protein capsids and two lipoprotein membranes German Andres, Diego Charro, Tania Matamoros, Rebecca Dillard, Nicola Abrescia
Journal Of Biological Chemistry, Oct 2019 DOI: 10.1074/jbc.AC119.011196 Architecture of human Rag GTPase heterodimers and their complex with mTORC1 Madhanagopal Anandapadamanaban, Glenn R. Masson, Olga Perisic, Alex Berndt, Jonathan Kaufman, Chris M. Johnson, Balaji Santhanam, Kacper B. Rogala, David M. Sabatini, Roger L. Williams
Science, 366 Oct 2019 DOI: 10.1126/science.aax3939 Journal Paper Krios I-Titan Krios I at Diamond Krios II-Titan Krios II at Diamond Structure of the inner kinetochore CCAN complex assembled onto a centromeric nucleosome Kaige Yan, Jing Yang, Ziguo Zhang, Stephen H. Mclaughlin, Leifu Chang, Domenico Fasci, Ann E. Ehrenhofer-Murray, Albert J. R. Heck, David Barford
Nature, 574 Oct 2019 DOI: 10.1038/s41586-019-1609-1 Journal Paper Krios I-Titan Krios I at Diamond Krios III-Titan Krios III at Diamond Krios V-Titan Krios V at Diamond open access The structure of bactofilin filaments reveals their mode of membrane binding and lack of polarity Xian Deng, Andres Gonzalez Llamazares, James Wagstaff, Victoria L. Hale, Giuseppe Cannone, Stephen H. Mclaughlin, Danguole Kureisaite-Ciziene, Jan Lowe
Nature Microbiology, 16 Sept 2019 DOI: 10.1038/s41564-019-0544-0 Journal Paper I03-Macromolecular Crystallography Krios I-Titan Krios I at Diamond Structural basis of light-induced redox regulation in the Calvin–Benson cycle in cyanobacteria Ciaran R. Mcfarlane, Nita R. Shah, Burak V. Kabasakal, Blanca Echeverria, Charles Cotton, Doryen Bubeck, James W. Murray
Proceedings Of The National Academy Of Sciences, 75 Sept 2019 DOI: 10.1073/pnas.1906722116 Journal Paper I02-Macromolecular Crystallography I03-Macromolecular Crystallography I04-Macromolecular Crystallography I24-Microfocus Macromolecular Crystallography Krios I-Titan Krios I at Diamond Krios III-Titan Krios III at Diamond open access Structural basis of Cullin 2 RING E3 ligase regulation by the COP9 signalosome Sarah V. Faull, Andy M. C. Lau, Chloe Martens, Zainab Ahdash, Kjetil Hansen, Hugo Yebenes, Carla Schmidt, Fabienne Beuron, Nora B. Cronin, Edward Morris, Argyris Politis
Nature Communications, 10 Sept 2019 DOI: 10.1038/s41467-019-11772-y Structures of influenza A virus RNA polymerase offer insight into viral genome replication Haitian Fan, Alexander P. Walker, Loic Carrique, Jeremy Keown, Itziar Serna Martin, Dimple Karia, Jane Sharps, Narin Hengrung, Els Pardon, Jan Steyaert, Jonathan M. Grimes, Ervin Fodor
Nature, 573 Sept 2019 DOI: 10.1038/s41586-019-1530-7 Journal Paper B21-High Throughput SAXS I03-Macromolecular Crystallography I24-Microfocus Macromolecular Crystallography Krios I-Titan Krios I at Diamond New structure for human flu virus protein Diamond Light Source
Sept 2019 Science Highlight B21-High Throughput SAXS I03-Macromolecular Crystallography I24-Microfocus Macromolecular Crystallography Krios I-Titan Krios I at Diamond
Atomic structure of the Epstein-Barr virus portal Cristina Machon, Montserrat Fàbrega-Ferrer, Daming Zhou, Ana Cuervo, José L. Carrascosa, David I. Stuart, Miquel Coll
Nature Communications, 10 Aug 2019 DOI: 10.1038/s41467-019-11706-8 Cyclin A2 degradation during the spindle assembly checkpoint requires multiple binding modes to the APC/C Suyang Zhang, Thomas Tischer, David Barford
Nature Communications, 10 Aug 2019 DOI: 10.1038/s41467-019-11833-2 Journal Paper Krios II-Titan Krios II at Diamond Krios III-Titan Krios III at Diamond open access Dimeric structures of quinol-dependent nitric oxide reductases (qNORs) revealed by cryo–electron microscopy Chai C. Gopalasingam, Rachel M. Johnson, George N. Chiduza, Takehiko Tosha, Masaki Yamamoto, Yoshitsugu Shiro, Svetlana V. Antonyuk, Stephen P. Muench, S. Samar Hasnain
Science Advances, 5 Aug 2019 DOI: 10.1126/sciadv.aax1803 Structure of the dynein-2 complex and its assembly with intraflagellar transport trains Katerina Toropova, Ruta Zalyte, Aakash G. Mukhopadhyay, Miroslav Mladenov, Andrew Carter, Anthony J. Roberts
Nature Structural & Molecular Biology, 26 Aug 2019 DOI: 10.1038/s41594-019-0286-y Journal Paper Krios I-Titan Krios I at Diamond Krios II-Titan Krios II at Diamond Krios III-Titan Krios III at Diamond Krios IV-Titan Krios IV at Diamond Krios V-Titan Krios V at Diamond Three-dimensional architecture and surface functionality of coccolith base plates B. Marzec, J. M. Walker, M. Panagopoulou, Y. Jhons, D. Clare, A. Wheeler, M. P. Shaver, F. Nudelman
Journal Of Structural Biology, Aug 2019 DOI: 10.1016/j.jsb.2019.08.007 Structures of T7 bacteriophage portal and tail suggest a viral DNA retention and ejection mechanism Ana Cuervo, Montserrat Fàbrega-Ferrer, Cristina Machon, Jose Javier Conesa, Francisco J. Fernández, Rosa Pérez-Luque, Mar Pérez-Ruiz, Joan Pous, M. Cristina Vega, José L. Carrascosa, Miquel Coll
Nature Communications, 10 Aug 2019 DOI: 10.1038/s41467-019-11705-9 LAT1 (SLC7A5) and CD98hc (SLC3A2) complex dynamics revealed by single-particle cryo-EM George N. Chiduza, Rachel M. Johnson, Gareth S. A. Wright, Svetlana V. Antonyuk, Stephen P. Muench, S. Samar Hasnain
Acta Crystallographica Section D Structural Biology, 75 Jul 2019 DOI: 10.1107/S2059798319009094 Plant-made nervous necrosis virus-like particles protect fish against disease Johanna Marsian, Daniel L. Hurdiss, Neil A. Ranson, Anneli Ritala, Richard Paley, Irene Cano, George P. Lomonossoff
Frontiers In Plant Science, 10 Jul 2019 DOI: 10.3389/fpls.2019.00880 Cryo-EM of dynein microtubule-binding domains shows how an axonemal dynein distorts the microtubule Samuel E. Lacey, Shaoda He, Sjors H. W. Scheres, Andrew P. Carter
Elife, 8 Jul 2019 DOI: 10.7554/eLife.47145 Cryo-EM and directed evolution reveal how Arabidopsis nitrilase specificity is influenced by its quaternary structure Andani Mulelu, Angela Kirykowicz, Jeremy Woodward
Communications Biology, 2 Jul 2019 DOI: 10.1038/s42003-019-0505-4 Structural insights into the activation of ATM kinase Jianxiong Xiao, Mengjie Liu, Yilun Qi, Yuriy Chaban, Chao Gao, Beiqing Pan, Yuan Tian, Zishuo Yu, Jie Li, Peijun Zhang, Yanhui Xu
Cell Research, 29 Jul 2019 DOI: 10.1038/s41422-019-0205-0 Journal Paper Krios II-Titan Krios II at Diamond Krios III-Titan Krios III at Diamond Krios IV-Titan Krios IV at Diamond Cryo-EM structures show how vertical single β-barrel viruses manage self-assembly Diamond Light Source
Jul 2019 Science Highlight Krios I-Titan Krios I at Diamond
Cryo‐EM structure of the MinCD copolymeric filament from Pseudomonas aeruginosa at 3.1 Å resolution Andrzej Szewczak-Harris, James Wagstaff, Jan Lowe
Febs Letters, June 2019 DOI: 10.1002/1873-3468.13471 A novel druggable interprotomer pocket in the capsid of rhino- and enteroviruses Rana Abdelnabi, James A. Geraets, Yipeng Ma, Carmen Mirabelli, Justin W. Flatt, Ausra Domanska, Leen Delang, Dirk Jochmans, Timiri Ajay Kumar, Venkatesan Jayaprakash , Barij Nayan Sinha, Pieter Leyssen, Sarah Butcher, Johan Neyts
Plos Biology, June 2019 DOI: 10.1371/journal.pbio.3000281 Two-step activation mechanism of the ClpB disaggregase for sequential substrate threading by the main atpase motor Celia Deville, Kamila Franke, Axel Mogk, Bernd Bukau, Helen Saibil
Cell Reports, 27 June 2019 DOI: 10.1016/j.celrep.2019.05.075 Structural and functional analysis of the role of the chaperonin CCT in mTOR complex assembly Jorge Cuellar, W. Grant Ludlam, Nicole C. Tensmeyer, Takuma Aoba, Madhura Dhavale, César Santiago, M. Teresa Bueno-Carrasco, Michael J. Mann, Rebecca L. Plimpton, Aman Makaju, Sarah Franklin, Barry M. Willardson, José M. Valpuesta
Nature Communications, 10 June 2019 DOI: 10.1038/s41467-019-10781-1 Structure of the DNA-bound spacer capture complex of a Type II CRISPR-Cas system Martin Wilkinson, Gediminas Drabavicius, Arunas Silanskas, Giedrius Gasiunas, Virginijus Siksnys, Dale B. Wigley
Molecular Cell, May 2019 DOI: 10.1016/j.molcel.2019.04.020 Journal Paper Krios II-Titan Krios II at Diamond Krios III-Titan Krios III at Diamond open access Multiple liquid crystalline geometries of highly compacted nucleic acid in a dsRNA virus Serban L. Ilca, Xiaoyu Sun, Kamel El Omari, Abhay Kotecha, Felix De Haas, Frank Dimaio, Jonathan M. Grimes, David I. Stuart, Minna M. Poranen, Juha T. Huiskonen
Nature, 22 May 2019 DOI: 10.1038/s41586-019-1229-9 The structural basis of translational control by eIF2 phosphorylation Tomas Adomavicius, Margherita Guaita, Yu Zhou, Martin D. Jennings, Zakia Latif, Alan Roseman, Graham D. Pavitt
Nature Communications, 10 May 2019 DOI: 10.1038/s41467-019-10167-3 Mechanism of completion of peptidyltransferase centre assembly in eukaryotes Vasileios Kargas, Pablo Castro-Hartmann, Norberto Escudero-Urquijo, Kyle Dent, Christine Hilcenko, Carolin Sailer, Gertrude Zisser, Maria J. Marques-Carvalho, Simone Pellegrino, Leszek Wawiórka, Stefan M. V. Freund, Jane L. Wagstaff, Antonina Andreeva, Alexandre Faille, Edwin Chen, Florian Stengel, Helmut Bergler, Alan John Warren
Elife, 8 May 2019 DOI: 10.7554/eLife.44904 A tail-based mechanism drives nucleosome demethylation by the LSD2/NPAC multimeric complex Chiara Marabelli, Biagina Marrocco, Simona Pilotto, Sagar Chittori, Sarah Picaud, Sara Marchese, Giuseppe Ciossani, Federico Forneris, Panagis Filippakopoulos, Guy Schoehn, Daniela Rhodes, Sriram Subramaniam, Andrea Mattevi
Cell Reports, 27 Apr 2019 DOI: 10.1016/j.celrep.2019.03.061 Journal Paper Krios I-Titan Krios I at Diamond Krios II-Titan Krios II at Diamond open access Physical basis for the loading of a bacterial replicative helicase onto DNA Ernesto Arias-Palomo, Neha Puri, Valerie L. O’shea Murray, Qianyun Yan, James M. Berger
Molecular Cell, 74 Apr 2019 DOI: 10.1016/j.molcel.2019.01.023 Structural basis for assembly of vertical single β-barrel viruses Isaac Santos-Perez, Diego Charro, David Gil-Carton, Mikel Azkargorta, Felix Elortza, Dennis H. Bamford, Hanna M. Oksanen, Nicola G. A. Abrescia
Nature Communications, 10 Mar 2019 DOI: 10.1038/s41467-019-08927-2 Architecture of the heteromeric GluA1/2 AMPA receptor in complex with the auxiliary subunit TARP γ8 Beatriz Herguedas, Jake F. Watson, Hinze Ho, Ondrej Cais, Javier Garcia-Nafria, Ingo H. Greger
Science, Mar 2019 DOI: 10.1126/science.aav9011 Novel tau filament fold in chronic traumatic encephalopathy encloses hydrophobic molecules Benjamin Falcon, Jasenko Zivanov, Wenjuan Zhang, Alexey G. Murzin, Holly J. Garringer, Ruben Vidal, R. Anthony Crowther, Kathy L. Newell, Bernardino Ghetti, Michel Goedert, Sjors H. W. Scheres
Nature, 91 Mar 2019 DOI: 10.1038/s41586-019-1026-5 In situ and high‐resolution cryo‐EM structure of a bacterial type VI secretion system membrane complex Chiara Rapisarda, Yassine Cherrak, Romain Kooger, Victoria Schmidt, Riccardo Pellarin, Laureen Logger, Eric Cascales, Martin Pilhofer, Eric Durand, Remi Fronzes
The Embo Journal, Mar 2019 DOI: 10.15252/embj.2018100886 The structure of a prokaryotic viral envelope protein expands the landscape of membrane fusion proteins Kamel El Omari, Sai Li, Abhay Kotecha, Thomas S. Walter, Eduardo A. Bignon, Karl Harlos, Pentti Somerharju, Felix De Haas, Daniel K. Clare, Mika Molin, Felipe Hurtado, Mengqiu Li, Jonathan Grimes, Dennis H. Bamford, Nicole D. Tischler, Juha T. Huiskonen, Dave I. Stuart, Elina Roine
Nature Communications, 10 Feb 2019 DOI: 10.1038/s41467-019-08728-7 Journal Paper I02-Macromolecular Crystallography I24-Microfocus Macromolecular Crystallography Krios I-Titan Krios I at Diamond open access Heparin-induced tau filaments are polymorphic and differ from those in Alzheimer’s and Pick’s diseases Wenjuan Zhang, Benjamin Falcon, Alexey G. Murzin, Juan Fan, R. Anthony Crowther, Michel Goedert, Sjors H. W. Scheres
Elife, 8 Feb 2019 DOI: 10.7554/eLife.43584 A human postcatalytic spliceosome structure reveals essential roles of metazoan factors for exon ligation Sebastian M. Fica, Chris Oubridge, Max E. Wilkinson, Andrew J. Newman, Kiyoshi Nagai
Science, 363 Feb 2019 DOI: 10.1126/science.aaw5569 Structure-function study of heteromeric amino acid transporter, LAT1-CD98hc George Nyasha Chiduza
Feb 2019 Thesis Krios III-Titan Krios III at Diamond
GABAA receptor signalling mechanisms revealed by structural pharmacology Simonas Masiulis, Rooma Desai, Tomasz Uchanski, Itziar Serna Martin, Duncan Laverty, Dimple Karia, Tomas Malinauskas, Jasenko Zivanov, Els Pardon, Abhay Kotecha, Jan Steyaert, Keith W. Miller, A. Radu Aricescu
Nature, 565 Jan 2019 DOI: 10.1038/s41586-018-0832-5