In the framework of the iNEXT-Discovery infrastructure https://inext-discovery.eu, the access providing partners IGBMC/CBI (Illkirch, France), EMBL-Heidelberg (Germany), CEITEC (Brno, Czeck Republic) and eBIC/Diamond (Oxford, UK) organise a 5-day hands-on workshop on cryo-FIB lamella preparation and cryo electron tomography.
Organising format: Distributed workshop with practicals at iNEXT-Discovery partner sites in parallel sessions and shared morning lectures (on-site + visio presentations) followed by all the participants at each site.
Aim: To provide hands-on training and discussion / experience feedback during practicals, lectures with general background and specific technical description on cellular sample preparation, FIB-milling and lamella preparation, cryo electron tomography data collection and initial quality assessment.
The workshop is open to PhD students, young scientists and engineer/facility staff. The number of places being limited, the participants will be selected on the basis of their applications. It is expected that applicants have a background in imaging, electron microscopy and structural biology, ideally with some experience with FIB and/or electron tomography. Also, we expect applicants to have the possibility to apply their newly acquired knowledge at their home institution (i.e. they have access to the relevant instruments, or will be applying to go to one of the centres). The applications comprising these informations will be reviewed and validated as they arrive and candidates informed of the status of their applications.
The workshop is free of charge and includes accommodation (from Sunday 14 to Friday 19, April), lunches and 2 dinners.
eBIC
