A Diamond–Charles Sturt Partner PhD Studentship
Diamond Supervisors (and respective beamlines): David Aragao (I04); Nathan Cowieson (B21)
The Scientific Premise
The nucleus of a cell acts as the library where the genomic information to build most of our protein micro-machines is stored. This library is well guarded, and a set of mechanisms drives the import and export of molecules into it. It is known how a virus can hijack such mechanisms not only to import their genomic information for incorporation into the cell “books” but also to bring in specialised proteins to interfere with immune response (e.g. blocking the DNA related to interferon transcription). On the other hand, defects in the mechanisms of import and export from the nucleus can lead to deregulation of gene expression programmes, often resulting in cancers. In this research project, we aim to shine light onto one of the mechanisms viruses use to suppress the innate immune response where viral proteins are transported into the human cell nucleus while bound to nuclear import receptors. The nuclear import recognition mechanism viral hijack is like tricking the elevator janitor that recognises cargo needed to go in and out of the nucleus via the nuclear membrane. Understanding at the molecular level how the cargo binds and interacts with the receptor is key to tackling certain infections.
With our comprehensive study, we hope to provide answers on why and how these diseases develop and possibly forge a path to combat such debilitating outcomes.
Training for the PhD Candidate
This will include specialised instruction in Python programming, all aspects of protein production and crystallisation as well as advanced use of large research facilities while at the Diamond Light Source synchrotron. At the latter, training in instrumentation, all areas of protein crystallography and small-angle X-ray scattering will additionally be provided through one-on-one sessions with two experts in the respective areas.
Time Division
The student will spend 50% of their time at Charles Sturt University and 50% at Diamond Light Source Ltd, the UK’s national synchrotron science facility, located at the Harwell Science and Innovation Campus in Oxfordshire. Please note that the successful applicant will be based in each institution for two years with no option to work fully remotely.
Attributes of Suitable Applicants
Required Skills
(1) Independent Worker: Ability to independently plan, manage and execute tasks completion. This includes setting realistic goals, prioritising tasks and troubleshooting problems effectively.
(2) Team Player: Strong interpersonal skills and a collaborative spirit to work effectively within a research team, share knowledge and contribute to a positive and productive lab environment.
(3) Scientific Curiosity: A genuine interest in scientific research and a desire to learn and explore new concepts and methodologies in biochemistry.
(4) Biochemistry Lab Experience: Experience working in a biochemistry lab
Additional, Desired Skills
(1) Experience with data analysis and scientific software (e.g. Spreadsheets, R, Python, others).
(2) Excellent written and verbal communication skills.
(3) Ability to document research methods and findings accurately and concisely.
(4) Strong work ethic and a commitment to deadlines.
(5) Laboratory proficiency in a variety of techniques such as protein purification, electrophoresis and biochemical assays, others.
Important Notes
This project is jointly funded for four years by Charles Sturt University and Diamond Light Source Ltd. Successful students will receive a stipend of no less than the standard UKRI stipend rate, currently set at £18,622 for the academic year 2023 to 2024 (the rates for subsequent academic years have not been published), plus a £2,000 per annum stipend top-up. Full funding is only available to qualified applicants who are eligible for home fees; applicants subject to international fees will be required to fund the difference between the home and international tuition fee rates (approximately £12,697 for the 2023/24 academic year).
Diamond Light Source is the UK's national synchrotron science facility, located at the Harwell Science and Innovation Campus in Oxfordshire.
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